Ethidium Bromide 

• Step 1 : Open the bottle cap of the sprayer and insert the sprayer head. The sprayer head has to be screwed onto the bottle tightly.
• Step 2 :  Spray onto the contaminated target area. The liquid has to completely cover the entire contaminant.
• Step 3 : Spray onto the bench for a) general protection of potential contaminated area. Spray the whole working area and wipe down after 5 minutes b) For known contaminated area. Spray on the specific working area on the following working time (see protocol)
• Step 4 : Please use the portable UV light box (UV Wavelength 254nm) to ensure the Ethidium Bromide has been totally destroyed.

SYBR Green I

• Spray on bench : Use the same procedure as for the Ethidium Bromide working area. However please note that 1.) The decontamination time is different to that of EtBr containing gels and 2.) You must use UV light box (wavelength 488nm) to observe SYBR Green.
• Spray on gels stained with SYBR Green : The procedure is the same as that of the Ethidium Bromide containing gels. However please note that 1.) The decontamination time is different to that of EtBr containing gels . For example, if you spray 0.9ml (about 3 sprays) on the gel stained with SYBR Green (concentration 0.5~1μg/ml), then after 1 hour, the SYBR Green will have been destroyed. Please use the UV light box (wavelength 488nm) to observe the SYBR Green.

SYBR Gold

• Spray on bench : Use the same procedure as for the Ethidium Bromide working area. However please note that 1.) The decontamination time is different to that of EtBr containing gels and 2.) You must use UV light box (wavelength 488nm) to observe SYBR Gold. 
• Spray on bench : Use the same procedure as for the Ethidium Bromide working area. However please note that 1.) The decontamination time is different to that of EtBr containing gels and 2.) You must use UV light box (wavelength 488nm) to observe SYBR Gold. Spray on gels stained with SYBR Gold : The procedures are the same as the usage for Ethidium Bromide containing gels. However please note that 1.) The decontamination time is different to that of EtBr containing gels. For example, if you spray 0.9ml (about 3 sprays) on the gel stained with SYBR Gold (concentration 0.5~1μg/ml), then after 1 hour, the SYBR Gold will have been destroyed. Please use the UV light box (wavelength 488nm) to observe the SYBR Gold.

SYPRO Orange

• Spray on bench : Use the same procedure as for the Ethidium Bromide working area. However please note that 1.) The decontamination time is different to that of EtBr containing gels and 2.) You must use UV light box (wavelength 488nm) to observe SYBR Orange. 
• Spray on gels stained with SYPRO Orange : The procedure is the same as that of the Ethidium Bromide containing gels. However please note that 1.) The decontamination time is different to that of EtBr containing gels. For example, if you spray 5.4ml (about 18 sprays) on the gel stained with SYPRO Orange (concentration 0.5~1μg/ml), then after 3 hours, the SYPRO Orange will have been destroyed. Please use the UV light box (wavelength 488nm) to observe the SYPRO Orange. 

ALPHAGEN EtBr Destroyer is provided in two different formats for the treatment of both solid and liquid Ethidium Bromide contaminant.

The ready pack ALPHAGEN EtBr Destroyer Bag is idea for the treatment of liquid Ethidium Bromide contaminant while Sprayer can be used for the treatment of solid contaminant waste including electrophoresis gels, glassware, paper towels, gloves, laboratory equipment, bench surface etc.

• Effective: ALPHAGEN EtBr Destroyer is a laboratory reagent intended for the removal and destruction of Ethidium Bromide contamination. This effect can be monitored and confirmed by UV light exposure whereby once the EtBr has been destroyed the fluorescence will disappear.
• Fast: For general protection of uncontaminated area, spray the EtBr Destroyer on the entire working area, leave for about 5 minutes, then wipe it dry with paper towel. When dealing with more seriously contaminated liquid samples the EtBr Destroyer bag is an ideal solution. Three litters of double distilled H2O containing 30mg of Ethidium Bromide can be destroyed in a matter of days using ALPHAGEN easy-to-follow up protocol with the bag format.
• Specific: Two specific designs are for different purposes : Ethidium Bromide Destroyer bags and Ethidium Bromide Destroyer Spray
•  Economical: ALPHAGEN EtBr Destroyer Sprayer bottles can be refilled after use and the bags are available in both a 10 and 20 pack. In comparison to the costs associated with properly disposing of hazardous materials the EtBr Destroyer products save your money in the long run. You can even re-use your buffer after treatment! One of the big economical benefits of using this product is that after using the ALPHAGEN EtBr Destroyer, the oncecontaminated buffer can be filtrated and use again without worry. 5. Safe: ALPHAGEN EtBr Destroyer has been demonstrated by Ames test to safely and effectively destroy EtBr without any mutagenic effect. The AMES test result data supports that the genotoxicity of EtBr contaminated solution, treated by both the ALPHAGEN EtBr Destroyer Band and Sprayer was reduced significantly and a negative result was determined.