α+ SolutionTM
Viral Nucleic Acid Extraction Kit

APVNK 004 4 preps_sample
APVNK 050 50 preps

Store at room temperature (15~ 25 ℃) for 2 year.
Except Carrier RNA Powder, store at -20℃

APVNK 004
4 preps
APVNK 050
50 preps
VNE Buffer 1.8 ml x 2 35ml
Wash Buffer 1 * (concentrate) 0.9 ml x 2 22 ml
Wash Buffer 2 * (concentrate) 1.5 ml 20 ml
RNase-free Water 0.5 ml 6 ml
Carrier RNA 0.05 mg 0.4 mg
VNE Column 4 pcs 50 pcs
Collection Tube 8 pcs 100 pcs
Elution Tube 4 pcs 50 pcs
User Manual 1 1
ethanol volume
for Wash Buffer 1
0.33 ml 8 ml
ethanol volume
for Wash Buffer 2
6 ml 80 ml

  • Principle : spin column (silica membrane)
  • Sample size : 150 µl cell-free fluid such as serum, plasma, body fluid and cell cultured supernatant 
  • Fragment size : 100 bp ~30 kb
  • Recovery rate : 80 ~ 90 %
  • Binding capacity : 30 ug
  • Elution volume : 40 ~ 50 µl 
  • Operation time : 20 min

Important Notes:

  • Make sure everything is RNase-free when handling this system.
  • Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
  • Add 1 ml of VNE Buffer to the tube of lyophilized Carrier RNA, mix well by vortexing and transfer the mixture to the VNE Buffer when first open. Store the Carrier RNA added VNE Buffer at 4 °C.
  • Add required ethanol (96-100%) to Wash Buffer 1 and Wash Buffer 2 before use. 5. Preheat RNase-free water to 70˚C for elution step.

 

 

Problems

Possible reasons

Solutions

Low or no yield of genomic DNA

 

Incorrect preparation of Wash Buffer 1 or Wash Buffer 2

Wash Buffer 1 and Wash Buffer 2 is not mixed with ethanol before use

Make sure that the correct volumes of ethanol (96- 100 %) is added into Wash Buffer 1 and Wash Buffer 2 when first open. Repeat the extraction procedure with a new sample.

The volume or the percentage of ethanol is not correct before adding into Wash Buffer 1 and Wash Buffer 2

Make sure that the correct volumes of ethanol (96- 100

%) is added into Wash Buffer 1 and Wash Buffer 2 when first use. Repeat the extraction procedure with a new sample.

 

Elution of genomic DNA is not efficient

RNase-free water not completely absorbed by column membrane

After RNase-free water is added, stand the VNE Column for 2 min before centrifugation.

Column is clogged

 

Sample is too viscous

Reduce the sample volume.

Degradation of eluted DNA

 

Sample is old

Always use fresh or well-stored sample viral nucleic acid extraction.

E-mail: sales@alphagen.bio
Mobile:+886-982-951-501
TEL:+886-8-736-7106
FAX:+886-8-736-7152

Address

No. 82, Ln. 11, Tantou Rd., Changzhi Township, Pingtung County 908, R.O.C.

Dsitributor

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