Tri-RNA Reagent is a reagent from the improved phenol and guanidine isothiocyanate (GSN) method for the single-step RNA isolation.During sample homogenization or lysis, Tri-RNA reagent maintains the integrity of the RNA, while disrupting cells and dissolving cell components.The composition of Tri-RNA reagent includes phenol and GSN in a mono-phase solution.A biological sample is homogenized or lysed in Tri-RNA reagent and homogenate is separated into the aqueous and organic phases by chloroform addition, vortexing, and centrifugation.RNA remains exclusively in the aqueous phase (clear upper phase), DNA in the interphase, and proteins remain in the organic phase (red color).RNA is precipitated from aqueous phase by addition of isopropanol and solubilized/concentrated.If needed, DNA and proteins can be sequentially precipitated from the interphase and organic phase with ethanol and isopropanol respectively, and solubilized/concentrated.
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