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EtBr Destroyer DNA Clean-Up DNA Extraction Plasmid Extraction RNase Destroyer RNA Extraction Straight PCR Viral Nucleic Acid
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APGCK004, APGCK050, APGCK100, APGCK300
α+ SolutionTM
GEL/ PCR Purification Kit

APGCK 004 4 preps
APGCK 050 50 preps
APGCK 100 100 preps
APGCK 300 300 preps

Protocol Order now
Component Brief Prodecure Specification Trouble shooting
APGCK 004 APGCK 050 APGCK 100 APGCK 300
DF Buffer 1.5 ml x 2 40 ml 80 ml 240 ml
Wash Buffer (concentrate) 1.0 ml 15 ml 25 ml 50 ml
Elution Buffer 0.5 ml 5 ml 6 ml 30 ml
DF Column 4 pcs 50 pcs 100 pcs 300 pcs
Collection Tube 4 pcs 50 pcs 100 pcs 300 pcs
User Manual 1 1 1 1

Specification:

  • Principle : spin column (silica matrix)
  • DNA Binding capacity of spin column : 20 µg
  • Sample size : up to 300 mg of agarose gel . up to 300 mg of agarose gel
  • DNA size : 65 bp ~ 10 kbp
  • Recovery : 70% ~ 85% for Gel extraction . 90% ~ 95% for PCR clean-up
  • Binding capacity : 10 ~ 20 min
  • Operation time : 40 µl

Important Notes:

  • Buffer provided in this kit contain irritants. Wear gloves and lab coat when handling these buffer.
  • Add the required volume of ethanol (96~100%) to Wash Buffer before use.
  • Centrifugation steps are done by a microcentrifuge capable of the speed at 11,000 ~1,8000 x g.

 

 

 

For Gel Extraction

Problems

Possible reasons

Solutions

The gel slice is hard to dissolve

Agarose gel of high percentage (> 2 %) is used

Add 1000 µl of DF Buffer to 1 volume of the gel slice.

The size of the gel slice is too large

If the gel slice is more than 300 mg, separate it into multiple tubes.

Low or none recovery of DNA fragment

The column is loaded with too much agarose gel

The maximum volume of the gel slice is 300 mg per column.

Elution of DNA fragment is not efficient

Make sure the pH of Elution Buffer or ddH2O is between 7.0- 8.5.

Make sure that the elution solution has been completely absorbed by the membrane before centrifuge.

The size of DNA fragment is larger than 5 Kb

Preheat the elution solution to 60 °C before use.

Eluted DNA contains non-specific

DNA fragment

Contaminated scalpel

Using a new or clean scalpel.

DNA fragment is denatured.

Incubate eluted DNA at 95 °C for 2 min, then cool down slowly to reanneal denatured DNA.

Poor perfor- mance in the downstream applications

Salt residue remains in eluted DNA fragment

Wash the column twice with Wash Buffer.

Ethanol residue remains in eluted DNA fragment

Do discard the flow-through after washing with Wash Buffer and centrifuge for an additional 3 min.

 

For PCR Clean-Up

Problems

Possible reasons

Solutions

Low or none recovery of DNA fragment

Apply more than 100 µl of PCR product

If the PCR product is more than 100 µl, separate it into multiple tubes.

Elution of DNA fragment is not efficient

Make sure the pH of Elution Buffer or ddH2O is between 7.0- 8.5.

Make sure that the elution solution has been completely absorbed by the column membrane before centrifuga- tion.

The size of DNA fragment is larger than 5 Kb

Preheat the elution solution to 60 °C before use.

Poor perfor- mance in the downstream applications

Salt residue remains in eluted DNA

Wash the column twice with Wash Buffer.

Ethanol residue remains in eluted DNA

Do discard the flow-through after washing with Wash Buffer and centrifuge for an additional 3 min.

 

 

E-mail: sales@alphagen.bio
Mobile:+886-982-951-501
TEL:+886-8-736-7106
FAX:+886-8-736-7152

Address

No. 82, Ln. 11, Tantou Rd., Changzhi Township, Pingtung County 908, R.O.C.

Dsitributor

ALPHAGEN Biotech will establish distributors in Japan, Korea, India, Europe, Pakistan, China & USA and covers 30+ countries with dedicated distributors.

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EtBr Destroyer DNA Clean-Up DNA Extraction Plasmid Extraction RNase Destroyer RNA Extraction Straight PCR Viral Nucleic Acid
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