APTRK 004 4 preps |
APTRK 050 50 preps |
APTRK 100 200 preps |
|
RB Buffer | 1.5 ml x 2 | 25 ml | 45 ml |
Wash Buffer 1 | 1.5 ml x 2 | 30 ml | 60 ml |
Wash Buffer 2 * (concentrate) | 1.5 ml | 15 ml | 35 ml |
RNase-free Water | 0.5 ml | 6 ml | 6 ml |
Filter Column | 4 pcs | 50 pcs | 100 pcs |
RB Mini Column | 4 pcs | 50 pcs | 200 pcs |
Collection Tube | 8 pcs | 100 pcs | 200 pcs |
Elution Tube | 4 pcs | 50 pcs | 200 pcs |
User Manual | 1 | 1 | 1 |
※ Principle : mini spin column (silica matrix)
※ Sample size : Please see Table. Sample amount and Yield.
※ Operation time : 30 ~ 60 minutes
※ Binding capacity : up to 100 μg total RNA/ column
※ Expected yield : Please see Table. Sample amount and Yield.
※ Column applicability : centrifugation and vaccum
※ Minimum elution volume : 40 μl
Important Notes :
※ Make sure everything is RNase-free when handling RNA.
※ Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
※ Caution: ß-mercaptoethanol is hazardous to human health. perform the procedures involving RB Buffer or PRB Buffer in a chemical fume hood.
※ Add required volume of RNase-free ethanol (96~100%) to Wash Buffer 2 when first use.
※ Dilute RNase-free DNase 1 in reaction buffer (1M NaCl, 10 mM MnCl2, 20 mM Tris-HCl, pH 7.0 at 25ºC) to final conc. = 0.5 U/μl.
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