α+ SolutionTM
Blood / Cultured Cell Total RNA Mini Kit

APBRK004 4 preps_sample
APBRK050 50 preps
APBRK100 100 preps

Store at room temperature (15~ 25 ℃) for 2 year.

(4 preps)
(50 preps)
(100 preps)
RL Buffer 15 ml x 2 120 ml 240 ml
RB Buffer 1.5 ml x 2 25 ml 45 ml
Wash Buffer 1 1.5 ml x 2 30 ml 60 ml
Wash Buffer 2 * (concentrate) 1.5 ml 15 ml 35 ml
RNase-free Water 0.5 ml 6 ml 6 ml
Filter Column 4 pcs 50 pcs 100 pcs
RB Mini Column 4 pcs 50 pcs 100 pcs
Collection Tube 8 pcs 100 pcs 200 pcs
Elution Tube 4 pcs 50 pcs 100 pcs
User Manual 1 1 1

※ Principle : mini spin column (silica matrix)

※ Sample size 300 μl

※ Operation time : 30 ~ 60 minutes

※ Binding capacity : up to 100 μg total RNA / column

※ Expected yield : 1 μg

※ Column applicability : centrifugation and vaccum

※ Minimum elution volume : 40 μl


Important Notes :

※ Make sure everything is RNase-free when handling RNA.

※ Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.

※ Caution: ß-mercaptoethanol is hazardous to human health. perform the procedures involving RB Buffer or PRB Buffer in a chemical fume hood. 

※ Add required volume of RNase-free ethanol (96~100%) to Wash Buffer 2 when first use.

※ Dilute RNase-free DNase 1 in reaction buffer (1M NaCl, 10 mM MnCl2, 20 mM Tris-HCl, pH 7.0 at 25ºC) to final conc. = 0.5 U/μl.


Possible reasons


Low yield


Carryover of erythrocytes.

Incomplete removal of the supernatant will interfere with lysis and subsequent binding of RNA to the RB column, resulting in lower yield.

Too much starting sample used may cause insufficient lysis and homogenization.

In Step.1 sample preparation, reduce the amount of starting sample or increase the volume of RL Buffer.

Incorrect RNA elution step.

Ensure that Elution Buffer was added and absorbed to the center of the PD Column matrix.

Ethanol carryover

Ensure that centrifuge at ≥ 8000 x g (≥10,000 rpm) for 3 min to dry the RB column membrane in Step.6 Dry Column.

Incorrect preparation of Wash 2 Buffer

Ensure that the correct volume of ethanol (96 ~ 100 %) was added to Wash 2 Buffer before use.

RNA degraded


Age of blood.

Please use the fresh blood sample.

Residual DNA


DNA Contamination.

Please follow the Optional step to remove DNA.

RNA does not perform well in downstream experiments


Salt carryover during elution.

Ensure that Wash 2 Buffer is at room temperature (15–25°C).

Ethanol carryover.

Perform the step. 6 in the protocol (centrifuge at  ≥  8000 x g or  ≥ 10,000 rpm) to remove all traces of ethanol before eluting.

E-mail: sales@alphagen.bio


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