APTGK004, APTGK050, APTGK100, APTGK200
α+ SolutionTM
Tissue Genomic DNA Extraction Mini Kit

APTGK004 4 preps_sample
APTGK050 50 preps
APTGK100 100 preps
APTGK200 200 preps

※ Store at room temperature (15~ 25 ℃) for 2 year.
※ Except Proteinase K Powder, store at 4℃.

APTGK 004
(4 preps)
APTGK 050
(50 preps)
APTGK 100
(100 preps)
APTGK 200
(200 preps)
TG1 Buffer 1.5 ml 15 ml 30 ml 60 ml
TG2 Buffer 1.5 ml 15 ml 30 ml 60 ml
Proteinase K
(lyophilized)
1 mg 11 mg 11 mg x 2 11 mg x 4
GW Buffer
(concentrate)
1.3 ml 22 ml 44 ml 88 ml
Wash Buffer
(concentrate)
1 ml 10 ml 20 ml 40 ml
Elution Buffer 1 ml 15 ml 30 ml 60 ml
TG Mini Column 4 pcs 50 pcs 100 pcs 200 pcs
Collection Tube 8 pcs 100 pcs 200 pcs 400 pcs
Elution Tube 4 pcs 50 pcs 100 pcs 200 pcs
User Manual 1 1 1 1

※ Principle : mini spin column (silica matrix)

※ Sample size 1. Up to 25 mg animal tissue

                             2. 1.2 cm mouse tail

                             3. Up to 107 cultured cells

※ Operation time : 30 ~ 60 minutes

※ Binding capacity : up to 60 µg total DNA / column

※ Expected yield : 15 ~35 μg / prep

※ Column applicability : centrifugation and vaccum

※ Minimum elution volume : 50 μl

 

Important Notes :

Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.

Add 1.1 ml sterile ddH2O to Proteinase K tube to make a 10 mg/ml stock solution. Make sure that Proteinase K has been completely dissolved. Store the stock solution at 4 ℃ .

Add ethanol (96- 100 %) to GW Buffer and Wash Buffer when first open.

Prepare dry baths or water baths before the operation. One to 60℃ for step 2.1 and the other to 70℃ for step 2.2.

Preheat the Elution Buffer to 70℃ for step 7.

All centrifuge steps are done at full speed(~ 18,000 x g) in a microcentrifuge.

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FAX:+886-8-736-7152

Address

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