APPGK004, APPGK050, APPGK100
α+ SolutionTM
Plant Genomic DNA Extraction Mini Kit

APPGK004 4 preps_sample
APPGK050 50 preps
APPGK100 100 preps

※ Store at room temperature (15~ 25 ℃) for 2 year.
※ Except RNase A Powder, store at 4℃.

APPGK 004
(4 preps)
APPGK 050
(50 preps)
APPGK 100
(100 preps)
PG1 Buffer 2 ml 25 ml 55 ml
PG2 Buffer 1 ml 8 ml 15 ml
PG3 Buffer (concentrate) 1.5 ml 15 ml 30 ml
GW Buffer (concentrate) 0.8 ml 13 ml 26 ml
Wash Buffer (concentrate) 1.5 ml 15 ml 30 ml
Elution Buffer 1.5 ml 15 ml 30 ml
RNase A (lyophilized) 1.5 mg 22 mg 43 mg
Filter Column 4 pcs 50 pcs 100 pcs
PG Column 4 pcs 50 pcs 100 pcs
Collection Tube 8 pcs 100 pcs 200 pcs
User Manual 1 1 1

※ Principle : mini spin column (silica matrix)

※ Sample size : 1. Wet weight ≦ 100 mg

                            2. Dry weight ≦ 20 mg

※ Operation time : 30 ~ 60 minutes

※ Binding capacity : up to 60 μg total DNA / column

※ Expected yield : 5 ~ 40 μg /prep

※ Column applicability : centrifugation and vaccum

※ Minimum elution volume : 50 μl

 

Important Notes :

Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.

Check PG1 Buffer before use, Warm PG1 Buffer at 60℃ for 5 min if any precipitate formd.

Preheat dry baths or water baths to 65℃ before the operation.

Add required ethanol (96-100%) to PG3 Buffer, GW and Wash before use.

Store RNase A at -20℃ upon recipit of kit. Add sterile ddH2O to RNase A tube to make a 50 mg/ml stock solution. Vortex and make sure that RNase A has been completely dissolved. Store the stock solution at -20℃ .

All centrifuge steps are done at full speed(~ 18,000 x g) in a microcentrifuge.

E-mail: sales@alphagen.bio
Mobile:+886-982-951-501
TEL:+886-8-736-7106
FAX:+886-8-736-7152

Address

No. 82, Ln. 11, Tantou Rd., Changzhi Township, Pingtung County 908, R.O.C.

Dsitributor

ALPHAGEN Biotech will establish distributors in Japan, Korea, India, Europe, Pakistan, China & USA and covers 30+ countries with dedicated distributors.

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