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APBGR004, APBGR100, APBGR200
α+ SolutionTM
Blood / Cultured Cell Genomic DNA Extraction Mini Kit

APBGR004 4 preps_sample
APBGR100 100 preps
APBGR200 200 preps

Store at room temperature (15~ 25 ℃) for 2 year.

Protocol Order now
Component Brief Prodecure Specification Trouble shooting
APBGR 004
(4 preps)		 APBGR 100
(100 preps)		 APBGR 200
(200 preps)
RBC Lysis Buffer 7 ml 135 ml 270 ml
TG Buffer 1.5 ml 30 ml 60 ml
BG Buffer 1.5 ml 40 ml 80 ml
BW Buffer 1.5 ml x 2 45 ml 90 ml
Wash Buffer (concentrate) 1 ml 25 ml 35 ml
Elution Buffer 1.5 ml 30 ml 60 ml
BG Mini Column 4 pcs 100 pcs 200 pcs
Collection Tube 8 pcs 200 pcs 400 pcs
User Manual 1 1 1

※ Principle : mini spin column (silica matrix)

※ Sample size : 1. Up to 300 μl of Whole blood

                            2. Up to 200 μl of frozen blood

                            3. Up to 200 μl of buffy coat

                            4. Up to 1 x 107 of Cultured animal cells

                            5. Up to 1 x 109 of Cultured bacterial cells

※ Operation time : 30 minutes

※ Binding capacity : up to 60 μg total DNA / column

※ Expected yield : 4 ~ 8 μg / prep

※ Column applicability : centrifugation and vaccum

※ Minimum elution volume : 50 μl

 

Important Notes :

※ Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.

※ Add ethanol (96- 100 %) to Wash Buffer when first open.

Problems

Possible reasons

Solutions

Low or no yield of genomic DNA

Incorrect preparation of Wash Buffer.

Wash Buffer is not mixed with ethanol before use.

Make sure that the correct volumes of ethanol (96 - 100 %) is added into the Wash Buffer when first opened. 

The volume or the percentage of ethanol is not correct before adding it into the Wash Buffer.

Make sure that the correct volumes of ethanol (96 - 100 %) is added into the Wash Buffer when first used.

Elution of genomic DNA is not efficient.

The Elution Buffer is not completely absorbed by the column membrane.

After the Elution Buffer  is added, stand the BG Column for 2 min before centrifugation.

The pH value of Elution Buffer is not correct.

Make sure the pH value of Elution Buffer is between 7.5- 8.5.

Column is clogged

 

Sample is too viscous.

Reduce the sample volume.

Degradation of eluted DNA

 

Sample is old.

Always use fresh or well-stored sample viral nucleic acid extraction.

E-mail: sales@alphagen.bio
Mobile:+886-982-951-501
TEL:+886-8-736-7106
FAX:+886-8-736-7152

Address

No. 82, Ln. 11, Tantou Rd., Changzhi Township, Pingtung County 908, R.O.C.

Dsitributor

ALPHAGEN Biotech will establish distributors in Japan, Korea, India, Europe, Pakistan, China & USA and covers 30+ countries with dedicated distributors.

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